Abstract
The unique clostridial endo-beta-galactosidase (Endo-beta-Gal(GnGa)) capable of releasing the disaccharide GlcNAc alpha 1,4Gal from O-glycans expressed in the gastric gland mucous cell-type mucin has been crystallized. The crystal belongs to space group P6(3), with unit-cell parameters a = 160.4, c = 86.1 A. Under cryocooled conditions and using a synchrotron X-ray source, the crystals diffract to 1.82 A resolution. The asymmetric unit contains two or three molecules.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins / chemistry*
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Bacterial Proteins / metabolism*
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Clostridium perfringens / enzymology*
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Crystallization
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Crystallography, X-Ray
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Disaccharides / metabolism*
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Gastric Mucosa / cytology
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Gastric Mucosa / metabolism
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Glycoside Hydrolases* / chemistry
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Glycoside Hydrolases* / metabolism
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Humans
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Mucins / metabolism
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Sialoglycoproteins / metabolism
Substances
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Bacterial Proteins
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Disaccharides
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Mucins
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Sialoglycoproteins
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Glycoside Hydrolases
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keratan-sulfate endo-1,4-beta-galactosidase