Chronic hepatotoxicity of carbon tetrachloride in hsp-70 knock out mice

Exp Anim. 2004 Jan;53(1):27-30. doi: 10.1538/expanim.53.27.

Abstract

The chronic hepatotoxic effects of carbon tetrachloride (CCl(4)) in heat-shock protein (HSP) 70 knock out (HSP70-/-) mice were examined. After repeated intraperitoneal injections of CCl(4) for six weeks, the level of ALT and weight ratio of the liver to body were lower in HSP70-/- mice than in the control (WT) mice. The levels of HSP25 and HSP47 were lowered in HSP70-/- mice as compared with WT mice. The grades of hepatic necrosis and neutrophil infiltration were not significantly different between HSP70-/- and WT mice. The collagen content was not affected significantly by CCl(4) treatment.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine Transaminase / blood*
  • Animals
  • Blotting, Western
  • Body Weights and Measures
  • Carbon Tetrachloride / toxicity*
  • Electrophoresis, Polyacrylamide Gel
  • HSP47 Heat-Shock Proteins
  • HSP70 Heat-Shock Proteins / genetics*
  • Heat-Shock Proteins / metabolism
  • Liver / drug effects*
  • Liver / metabolism
  • Liver / pathology
  • Mice
  • Mice, Knockout
  • Molecular Chaperones
  • Neoplasm Proteins / metabolism
  • Neutrophil Infiltration
  • Neutrophils / pathology

Substances

  • HSP47 Heat-Shock Proteins
  • HSP70 Heat-Shock Proteins
  • Heat-Shock Proteins
  • Hsbp1 protein, mouse
  • Molecular Chaperones
  • Neoplasm Proteins
  • Serpinh1 protein, mouse
  • Carbon Tetrachloride
  • Alanine Transaminase