Abstract
Substituted 3-amino-2-hydroxyamides and related hydroxyamides and acylhydrazines were identified as inhibitors of human methionine aminopeptidase-2 (MetAP2). Examination of substituents through parallel synthesis and iterative structure-based design allowed the identification of potent inhibitors with good selectivity against MetAP1. Diacylhydrazine 3t (A-357300) was identified as an analogue displaying inhibition of methionine processing and cellular proliferation in human microvascular endothelial cells (HMVEC).
MeSH terms
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Amides / chemistry*
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Amides / pharmacology*
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Aminopeptidases / antagonists & inhibitors*
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Binding Sites / drug effects
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Cell Division / drug effects
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects
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Enzyme Inhibitors / chemistry*
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Enzyme Inhibitors / pharmacology*
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Humans
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Metalloendopeptidases / antagonists & inhibitors*
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Methionine / drug effects
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Models, Biological
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Models, Molecular
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Molecular Structure
Substances
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Amides
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Enzyme Inhibitors
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Methionine
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Aminopeptidases
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methionine aminopeptidase 2
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Metalloendopeptidases