Combined frequency- and time-domain NMR spectroscopy. Application to fast protein resonance assignment

J Biomol NMR. 2004 May;29(1):57-64. doi: 10.1023/B:JNMR.0000019501.21697.34.

Abstract

A simple and general method is presented to simplify multi-dimensional NMR spectra of isotope-labeled bio-molecules. The approach is based on band-selective Hadamard-type frequency encoding, which disperses the correlation peaks into different sub-spectra. This makes it possible to apply low-dimensionality-based NMR techniques to larger molecular systems. Here we demonstrate the use of band-selective Hadamard frequency labeling for fast protein resonance assignment, based on our recently proposed suite of 2D experiments (Bersch et al., 2003).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Models, Chemical
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Protein Conformation
  • Protein Structure, Tertiary
  • Proteins / chemistry*
  • Statistics as Topic
  • Time Factors

Substances

  • Proteins