Abstract
Sixteen of the 18 Vibrio cholerae rough strains isolated from hospitalised diarrhoea patients were found to contain O1 serotype-specific (wbe) genes and all currently known virulence genes. Expression of the regulatory element ToxR was evident in these strains. Cholera toxin production ability of the rough strains was found to be higher (c. three- to five-fold) as compared to the smooth counterparts and this was transcriptionally regulated. Strains exhibiting the rough phenotype were more amenable to the uptake of CTXphi, which led us to consider that the rough phenotype could play a role in the generation of genetic diversity among V. cholerae strains.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics
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Bacteriophages / genetics
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Bacteriophages / physiology
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Cholera / microbiology*
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Cholera Toxin / biosynthesis
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Cholera Toxin / genetics
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics
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Diarrhea / microbiology
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Feces / microbiology
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Gene Expression Regulation, Bacterial*
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Genes, Bacterial
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Genomic Islands / genetics
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Humans
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India
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Lysogeny
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Molecular Epidemiology
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O Antigens / analysis
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O Antigens / genetics
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Ribotyping
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Transcription Factors / biosynthesis
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Transcription Factors / genetics
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Transcription, Genetic
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Transduction, Genetic
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Vibrio cholerae / classification
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Vibrio cholerae / genetics*
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Vibrio cholerae / isolation & purification*
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Virulence Factors / analysis
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Virulence Factors / genetics
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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O Antigens
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Transcription Factors
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Virulence Factors
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toxR protein, Vibrio cholerae
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toxR protein, bacteria
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tcpN protein, Vibrio cholerae
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Cholera Toxin