Molecular characterization of the integrons in Shigella strains isolated from patients with traveler's diarrhea

Diagn Microbiol Infect Dis. 2004 Mar;48(3):175-9. doi: 10.1016/j.diagmicrobio.2003.10.007.

Abstract

The prevalence and characterization of Class 1 integrons has been performed in eighty three strains of Shigella spp., isolated between 1995 and 2000 from patients with traveler's diarrhea. A low prevalence (13.25%) was recorded. Nine different integrons were found among 11 multiresistant strains, with a total of 10 different gene cassettes encoding for resistance to trimethoprim (dfrA1, dfrA5, dfrA7, dfrA12, and dfrA15), aminoglycosides (aadA1a and aadA2), beta-lactam antibiotics (oxa2) or ORF with unknown function (orfD and orfF). A high prevalence of dfr and aad gene cassettes was observed. The low incidence of Class 1 integrons observed in this study is in contrast with the known facility that the Shigella genus has to gain and transfer plasmids.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Base Sequence
  • DNA, Bacterial / analysis
  • Drug Resistance, Multiple, Bacterial
  • Dysentery, Bacillary / drug therapy
  • Dysentery, Bacillary / epidemiology
  • Dysentery, Bacillary / genetics*
  • Humans
  • Integrons / genetics*
  • Microbial Sensitivity Tests
  • Molecular Biology
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Sensitivity and Specificity
  • Shigella / classification
  • Shigella / genetics*
  • Spain / epidemiology
  • Travel*

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial