Abstract
68Ge/68Ga generators provide cyclotron-independent access to positron emission tomography (PET) radiopharmaceuticals. We describe a system which allows the safe and efficient handling of 68Ge/68Ga generator eluates for labelling of DOTA-derivatised peptide ligands. The system comprises concentration and purification of the 68Ga eluate as well as labelling and purification steps for peptides, and can be used with different 68Ge/68Ga generator types. The suitability and efficiency were tested with two different DOTA-derivatised somatostatin derivatives and a DOTA-derivatised bombesin derivative. Amounts of 10-20 nmol of the peptides were sufficient and resulted in labelling yields of 50% for all peptides. The built-in safety precautions have proven to be appropriate in allowing use of the method for routine clinical applications. The system was set up and operated in a "hot lab" by personnel with no previous experience in the preparation of PET radiopharmaceuticals.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bombesin / analogs & derivatives
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Bombesin / isolation & purification
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Gallium Radioisotopes / chemistry*
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Gallium Radioisotopes / isolation & purification
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Heterocyclic Compounds, 1-Ring / chemical synthesis
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Heterocyclic Compounds, 1-Ring / chemistry*
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Heterocyclic Compounds, 1-Ring / isolation & purification
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Isotope Labeling / instrumentation*
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Isotope Labeling / methods*
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Ligands
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Peptides / chemical synthesis
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Peptides / chemistry*
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Peptides / isolation & purification
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Radiopharmaceuticals / chemical synthesis
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Radiopharmaceuticals / chemistry*
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Radiopharmaceuticals / isolation & purification
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Somatostatin / analogs & derivatives
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Somatostatin / isolation & purification
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Specimen Handling / instrumentation
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Specimen Handling / methods
Substances
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Gallium Radioisotopes
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Heterocyclic Compounds, 1-Ring
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Ligands
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Peptides
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Radiopharmaceuticals
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1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid
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Somatostatin
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Bombesin