Soils enzymes play an important role in the remediation of polluted soils. The enzymological remediation of polluted soils has the characteristics of high specialization, wide applicability, and little sensitivity to the ambient factors. This paper reviewed the advantages of enzymological remediation of polluted soils, the methods and technologies of enzyme immobilization, and the removal mechanisms of pollutants by oxidoreductases. The sources and characteristics of the enzymes used to remediation were also introduced, and some suggestions about the research in the future were put forward.