Abstract
Daxx has been reported to mediate the Fas/JNK-dependent signals in the cytoplasm. However, several evidences have suggested that Daxx is located mainly in the nucleus and functions as a transcriptional regulator. Recently, we identified DMAP1, a TSG101-interacting protein as a Daxx binding partner by yeast two-hybrid screening. TSG101 has been shown to act as transcriptional co-repressor of nuclear hormone receptors. Here we examined whether TSG101also interacts with Daxx directly. The association of Daxx and TSG101 was confirmed using co-expressed tagged proteins. The interaction regions in both proteins were also mapped, and the cellular localization of the interaction was examined. TSG101 formed a complex with Daxx through its coiled-coil domain and co-localized in the nucleus. Furthermore, TSG101 enhanced Daxx-mediated repression of glucocorticoid receptor transcriptional activity. These results provide the novel molecular interactions between Daxx and TSG101, which establish an efficient repressive transcription complex in the nucleus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Animals
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COS Cells
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Carrier Proteins / chemistry*
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Carrier Proteins / metabolism
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Cell Line
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Cell Nucleus / metabolism
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Co-Repressor Proteins
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Cytoplasm / metabolism
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DNA-Binding Proteins / chemistry*
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DNA-Binding Proteins / metabolism
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Dose-Response Relationship, Drug
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Endosomal Sorting Complexes Required for Transport
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Fluorescent Antibody Technique, Indirect
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Humans
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Immunoblotting
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Intracellular Signaling Peptides and Proteins*
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Luciferases / metabolism
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Molecular Chaperones
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Nuclear Proteins / chemistry*
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Nuclear Proteins / metabolism
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Precipitin Tests
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Protein Binding
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Protein Structure, Tertiary
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Receptors, Glucocorticoid / metabolism
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Transcription Factors / chemistry*
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Transcription Factors / metabolism
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Transcription, Genetic
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Transfection
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Two-Hybrid System Techniques
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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Co-Repressor Proteins
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DAXX protein, human
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DNA-Binding Proteins
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Endosomal Sorting Complexes Required for Transport
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Intracellular Signaling Peptides and Proteins
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Molecular Chaperones
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Nuclear Proteins
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Receptors, Glucocorticoid
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Transcription Factors
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Tsg101 protein
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Luciferases