Abstract
Aurora B is an important regulator of mitosis, and its mRNA and protein levels are tightly regulated during the cell cycle. In this study, we cloned the 5' flanking region of the human aurora B gene and characterized its promoter activity. Two major transcription initiation sites were identified by primer extension. aurora B promoter activity was upregulated during M phase, and its cell cycle-dependent element (CDE) and cell cycle-gene homology region (CHR) upstream of the transcription initiation sites regulated the cell cycle-dependent promoter activity. Several CDE-binding protein complexes were identified using the electrophoretic mobility shift assay. Using the biotin-streptavidin pull-down assay, binding of E2F-1, E2F-4, and DP-2, but not of DP-1, to the CDE was detected. These results demonstrate that aurora B mRNA level is regulated by CDE-CHR and that a subset of E2F/DP family proteins binds to the CDE.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aurora Kinase B
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Aurora Kinases
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Base Sequence
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Biotin / metabolism
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Cell Cycle Proteins*
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Cell Cycle*
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Cell Nucleus / metabolism
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DNA-Binding Proteins / metabolism
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E2F Transcription Factors
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E2F1 Transcription Factor
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E2F4 Transcription Factor
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HeLa Cells
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Humans
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Luciferases / metabolism
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Mitosis
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Molecular Sequence Data
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Plasmids / metabolism
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Promoter Regions, Genetic*
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Protein Binding
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Protein Serine-Threonine Kinases / genetics*
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Streptavidin / metabolism
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Transcription Factors / metabolism
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Transcription, Genetic
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Transfection
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Up-Regulation
Substances
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Cell Cycle Proteins
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DNA-Binding Proteins
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E2F Transcription Factors
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E2F1 Transcription Factor
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E2F1 protein, human
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E2F4 Transcription Factor
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E2F4 protein, human
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RNA, Messenger
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TFDP2 protein, human
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Transcription Factors
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Biotin
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Streptavidin
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Luciferases
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AURKB protein, human
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Aurora Kinase B
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Aurora Kinases
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Protein Serine-Threonine Kinases