Abstract
We investigated whether repression of JNK by hepatocyte growth factor/scatter factor (HGF/SF) in MDCK epithelial cells is linked to its ability to protect cells from apoptosis. To this purpose, cells were treated by TNF-alpha, a well-known inducer of JNK and of cell death, and the effects of HGF/SF were investigated under these conditions. We identified repression of JNK as a signaling target of HGF/SF for protection against TNF-alpha-induced cell death. This effect of HGF/SF occurs via the activation of the PI3K and MEK1 pathways.
MeSH terms
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Animals
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Apoptosis / drug effects
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Apoptosis / physiology*
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Cell Line
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Cell Survival / drug effects
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Chromones / pharmacology
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Dogs
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Enzyme Inhibitors / pharmacology
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Hepatocyte Growth Factor / pharmacology*
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JNK Mitogen-Activated Protein Kinases*
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MAP Kinase Kinase 4
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Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors*
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Morpholines / pharmacology
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Tumor Necrosis Factor-alpha / pharmacology*
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Urothelium
Substances
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Chromones
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Enzyme Inhibitors
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Morpholines
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Tumor Necrosis Factor-alpha
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2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
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Hepatocyte Growth Factor
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JNK Mitogen-Activated Protein Kinases
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MAP Kinase Kinase 4
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Mitogen-Activated Protein Kinase Kinases