Abstract
The VanA ligase encoded by the vancomycin resistance plasmid pIP816 of Enterococcus faecium BM4147 condenses D-alanine with various D-2-hydroxy and D-2-amino acids in vitro. D-Lactate added to the culture medium restored the vancomycin resistance of a strain that does not produce the VanH dehydrogenase and therefore appears to be a substrate of VanA in vivo.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Drug Resistance, Microbial
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Enterococcus faecium / drug effects
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Enterococcus faecium / metabolism*
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Lactates / metabolism*
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Lactic Acid
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Microbial Sensitivity Tests
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Peptidoglycan / metabolism*
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Vancomycin / metabolism
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Vancomycin / pharmacology
Substances
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Lactates
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Peptidoglycan
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Lactic Acid
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Vancomycin