Abstract
Inflammation is a significant component of chronic neurodegenerative diseases. Cyclooxygenase-2 (COX-2) is expressed in activated microglial cells and appears to be an important source of prostaglandins during inflammatory conditions. To investigate the effect of curcumin on COX-2 gene expression in microglial cells, we treated lipopolysaccharide (LPS)-challenged BV2 microglial cells with various concentrations of curcumin. Curcumin significantly inhibited LPS-mediated induction of COX-2 expression in both mRNA and protein levels in a concentration-dependent manner. COX-2 enzyme activity was also inhibited in accordance with mRNA and protein levels. Furthermore, curcumin markedly inhibited LPS-induced nuclear factor kappaB (NF-kappaB) and activator protein 1 (AP-1) DNA bindings. These data suggest that curcumin suppresses LPS-induced COX-2 gene expression by inhibiting NF-kappaB and AP-1 DNA bindings in BV2 microglial cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cells, Cultured*
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Curcumin / chemistry
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Curcumin / pharmacology*
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Cyclooxygenase 2
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Gene Expression / drug effects
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Gene Expression / genetics
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Lipopolysaccharides / antagonists & inhibitors*
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Lipopolysaccharides / metabolism
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Lipopolysaccharides / pharmacology
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Mice
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Microglia / cytology*
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Microglia / drug effects
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Microglia / physiology
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NF-kappa B / antagonists & inhibitors*
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NF-kappa B / drug effects
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NF-kappa B / metabolism
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Prostaglandin-Endoperoxide Synthases / genetics*
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Prostaglandin-Endoperoxide Synthases / metabolism
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Prostaglandins / metabolism
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RNA, Messenger / drug effects
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RNA, Messenger / metabolism
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Transcription Factor AP-1 / antagonists & inhibitors*
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Transcription Factor AP-1 / drug effects
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Transcription Factor AP-1 / metabolism
Substances
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Lipopolysaccharides
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NF-kappa B
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Prostaglandins
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RNA, Messenger
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Transcription Factor AP-1
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Cyclooxygenase 2
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Prostaglandin-Endoperoxide Synthases
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Curcumin