Somatostatin analogue treatment attenuates histological findings of inflammation and increases mRNA expression of interleukin-1 beta in the articular tissues of rats with ongoing adjuvant-induced arthritis

Rheumatol Int. 2005 Jun;25(5):350-6. doi: 10.1007/s00296-004-0455-z. Epub 2004 Mar 26.

Abstract

Objective: Somatostatin is a neuropeptide with modulatory effects on the immune system and the function of synovial cells; it has antiangiogenic and antiproliferative properties. This study aimed to evaluate the clinical, histological, and articular tissue cytokine mRNA response to somostatin treatment in rat adjuvant-induced arthritis (AIA).

Methods: Adjuvant-induced arthritis was induced in a total of 68 Lewis rats by immunization with complete Freund's adjuvant. Twenty-four rats were treated with a long-acting somostatin analogue 14 days after disease induction. Twenty-four untreated rats served as controls. The severity of arthritis was scored weekly for 42 days. In a second experiment, 20 rats (ten treated, ten controls) were killed 21 days after treatment for assessment of joint histopathology and articular tissue cytokine mRNA expression.

Results: Somatostatin analogue treatment significantly reduced histological scores of early inflammatory changes and increased articular tissue mRNA expression of interleukin-1 beta (IL-1beta). A trend toward improvement in physical scores of joint inflammation was seen in the treated group. Late destructive changes were not significantly different.

Conclusion: Treatment with a somostatin analogue attenuated early inflammatory changes in AIA joints and increased mRNA expression of IL-1beta in the articular tissues of rats with ongoing arthritis. Improvement in the physical findings of joint inflammation was mild.

MeSH terms

  • Animals
  • Arthritis, Experimental / drug therapy*
  • Arthritis, Experimental / metabolism
  • Arthritis, Experimental / pathology
  • Disease Models, Animal
  • Female
  • Gene Expression / drug effects
  • Injections, Intramuscular
  • Interleukin-1 / genetics
  • Interleukin-1 / metabolism*
  • Joints / drug effects*
  • Joints / metabolism
  • Joints / pathology
  • Octreotide / therapeutic use*
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Inbred Lew
  • Treatment Outcome

Substances

  • Interleukin-1
  • RNA, Messenger
  • Octreotide