Abstract
We investigated the effect of disodium cromoglycate (DSCG) on mast cell-mediated immediate-type hypersensitivity. DSCG inhibited systemic allergic reaction induced by compound 48/80 dose-dependently. Passive cutaneous anaphylaxis was inhibited by 71.6% by oral administration of DSCG (1 g/kg). When DSCG was pretreated at concentration rang from 0.01-1000 g/kg, the serum histamine levels were reduced in a dose dependent manner. DSCG also significantly inhibited histamine release from rat peritoneal mast cell (RPMC) by compound 48/80. We confirmed that DSCG inhibited compound 48/80-induced degranulation of RPMC by alcian blue/nuclear fast red staining. In addition, DSCG showed a significant inhibitory effect on anti-dinitrophenyl IgE-mediated tumor necrosis factor-alpha production. These results indicate that DSCG inhibits mast cell-mediated immediate-type allergic reaction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Administration, Oral
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Anaphylaxis / chemically induced
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Anaphylaxis / mortality
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Anaphylaxis / prevention & control*
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Animals
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Anti-Asthmatic Agents / administration & dosage
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Anti-Asthmatic Agents / pharmacology*
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Cell Degranulation / drug effects
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Cromolyn Sodium / administration & dosage
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Cromolyn Sodium / pharmacology*
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Dose-Response Relationship, Drug
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Histamine Release / drug effects*
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Longevity / drug effects
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Male
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Mast Cells / drug effects*
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Mast Cells / immunology
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Mast Cells / pathology
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Mice
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Mice, Inbred ICR
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Passive Cutaneous Anaphylaxis / drug effects
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Passive Cutaneous Anaphylaxis / immunology
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Peritoneal Cavity / cytology
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Rats
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Rats, Sprague-Dawley
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Survival Rate
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Tumor Necrosis Factor-alpha / metabolism
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p-Methoxy-N-methylphenethylamine / pharmacology
Substances
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Anti-Asthmatic Agents
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Tumor Necrosis Factor-alpha
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p-Methoxy-N-methylphenethylamine
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Cromolyn Sodium