Successful graft of HTLV-I-transformed human T-cells (MT-2) in severe combined immunodeficiency mice treated with anti-asialo GM-1 antibody

Jpn J Cancer Res. 1992 Apr;83(4):320-3. doi: 10.1111/j.1349-7006.1992.tb00108.x.

Abstract

To develop an experimental model of adult T-cell leukemia/lymphoma in small animals, severe combined immunodeficiency (SCID) mice treated with anti-asialo GM-1 antibody were inoculated with MT-2 cells, a cell line transformed by the human T-cell leukemia virus (HTLV-I). Three mice injected with 4 x 10(7) cells subcutaneously or intramuscularly developed tumors at or near inoculation sites. Immunofluorescent antibody (IFA) staining for HTLV-I structural protein, p19, revealed the specific antigen in the cytoplasm of most cells from tumors and the DNA signals of HTLV-I proviral DNA were also positive in cellular DNA by polymerase chain reaction assay with HTLV-I tax gene primers, SK43/SK44. The MT-2 cells did not invade in mouse organs.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies / administration & dosage*
  • Cell Line, Transformed
  • Cell Transformation, Viral*
  • DNA, Neoplasm / genetics
  • DNA, Neoplasm / isolation & purification
  • Fluorescent Antibody Technique
  • G(M1) Ganglioside / immunology*
  • Human T-lymphotropic virus 1 / genetics*
  • Humans
  • Leukemia, T-Cell / pathology*
  • Lymphoma, T-Cell / pathology*
  • Mice
  • Mice, SCID
  • Neoplasm Transplantation
  • Polymerase Chain Reaction
  • T-Lymphocytes / transplantation*
  • Transplantation, Heterologous

Substances

  • Antibodies
  • DNA, Neoplasm
  • G(M1) Ganglioside
  • asialo GM1 ganglioside