Objective: To study three-dimensional culturing methods of neonatal rat cardiac myocytes in simulated microgravity.
Methods: Neonatal rat primary cardiac myocytes were separated and seeded into polylactic acid scaffolds, stirred in spinner flasks for 24 hours, and then moved into rotary cell culture system for three-dimensional culture. The growth of cardiac myocytes was observed under inverted phase contrast microscope, scanning electron microscope and transmission electron microscope, and metabolic assay was assessed by MTT assay.
Results: Cardiac myocytes with sustained metabolic activity attached to the polylactic acid scaffolds, extended and confluenced. Pulsations of PLA-cardiac myocytes was found in some areas.
Conclusion: The rotary cell culture system is suitable to develop neonatal rat cardiac myocytes culturing for three-dimensional modeling.