Complete nucleotide sequence of a potyvirus causing maize dwarf mosaic disease in central China

Acta Virol. 2003;47(4):223-7.

Abstract

The full-length nucleotide sequence of a potyvirus causing the maize dwarf mosaic (MDM) disease in Henan province, central China, was obtained by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA 5'-end (5'-RACE). The viral genome comprised of 9596 nucleotides except the polyA tail and encoded a putative polyprotein of 3603 amino acids. The entire genomic sequence of this isolate shared identities of 94.2% and 98.3% with Sugarcane mosaic virus (SCMV) HZ isolate at the nucleotide and deduced amino acid levels, respectively, but only a 69.1% identity with MDM virus (MDMV) Bulgarian isolate (MDMV-Bg) at the nucleotide level. Phylogenetical tree analysis of the complete nucleotide sequences indicated that the Henan isolate of a potyvirus causing MDM disease is in fact a Henan strain of SCMV (SCMV-HN).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • China
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA, Viral / genetics
  • Genome, Viral
  • Molecular Sequence Data
  • Phylogeny
  • Plant Diseases / virology*
  • Potyvirus / classification
  • Potyvirus / genetics*
  • Potyvirus / pathogenicity*
  • Zea mays / virology*

Substances

  • DNA, Complementary
  • DNA, Viral

Associated data

  • GENBANK/AJ310106
  • GENBANK/AJ310107
  • GENBANK/AJ310110
  • GENBANK/AJ310117
  • GENBANK/AY042184