Application of ORAL.screen saliva drug test for the screening of methamphetamine, MDMA, and MDEA incorporated in hair

J Anal Toxicol. 2004 Mar;28(2):132-4. doi: 10.1093/jat/28.2.132.

Abstract

By the use of a one-step immunoassay drug test for oral fluid, a convenient and fairly sensitive screening method has been devised for methamphetamine (MA), 3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxyethylamphetamine (MDEA) incorporated in hair. These drugs, in a 10-mg portion of hair, were extracted into 5M HCl/methanol (1:20, v/v), and the extract reconstituted in 100 micro L water was assayed with the saliva drug test ORAL.screen trade mark. The limits of detection were 0.5 ng/mg hair for d-MA, 0.8 ng/mg for dl-MDMA, and 1.0 ng/mg for dl-MDEA. The results are in good agreement with those of gas chromatography-mass spectrometry (GC-MS) determination. Although all positive results must be confirmed by either GC-MS or a specific alternative methodology, this method provided a simple screening, suitable for drug enforcement purposes, while requiring only a 10-mg hair specimen.

MeSH terms

  • 3,4-Methylenedioxyamphetamine / analogs & derivatives*
  • 3,4-Methylenedioxyamphetamine / analysis*
  • Forensic Medicine / methods
  • Hair / chemistry*
  • Humans
  • Immunoassay
  • Immunochemistry
  • Methamphetamine / analysis*
  • N-Methyl-3,4-methylenedioxyamphetamine / analysis*
  • Reagent Kits, Diagnostic*
  • Saliva / chemistry
  • Substance Abuse Detection / methods*

Substances

  • Reagent Kits, Diagnostic
  • Methamphetamine
  • 3,4-Methylenedioxyamphetamine
  • N-Methyl-3,4-methylenedioxyamphetamine
  • 3,4-methylenedioxyethamphetamine