Abstract
We have conducted an international quality assurance study of filovirus, Lassa virus, and orthopox virus PCR with 24 participants. Of the participating laboratories, 45.8 and 66.7% detected virus in all plasma samples, which contained > or = 5,000 and > or = 100,000 copies per ml, respectively. Sensitivity levels were not significantly different between viruses. False-negative results were attributable to a lack of sensitivity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bioterrorism*
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DNA, Viral / blood
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Ebolavirus / genetics
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Ebolavirus / isolation & purification*
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Europe
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Humans
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International Cooperation*
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Laboratories
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Lassa virus / genetics
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Lassa virus / isolation & purification*
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Marburgvirus / genetics
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Marburgvirus / isolation & purification*
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Orthopoxvirus / genetics
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Orthopoxvirus / isolation & purification*
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Polymerase Chain Reaction / methods*
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Quality Control
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Virus Diseases / diagnosis
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Virus Diseases / virology