Abstract
A multiplex PCR for detection of three categories of diarrheagenic Escherichia coli was developed. With this method, enterohemorrhagic E. coli, enteropathogenic E. coli, and enterotoxigenic E. coli were identified in fecal samples from patients with hemorrhagic colitis, watery diarrhea, or hemolytic-uremic syndrome and from food-borne outbreaks.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Colitis / diagnosis
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Colitis / microbiology
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Diarrhea / diagnosis
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Diarrhea / microbiology*
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Escherichia coli / classification*
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Escherichia coli / genetics*
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Escherichia coli / isolation & purification
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Escherichia coli Infections / diagnosis*
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Escherichia coli Infections / microbiology
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Escherichia coli O157 / classification
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Escherichia coli O157 / genetics
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Escherichia coli O157 / isolation & purification
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Escherichia coli Proteins / genetics
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Feces / microbiology
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Foodborne Diseases / diagnosis
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Foodborne Diseases / microbiology
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Hemolytic-Uremic Syndrome / diagnosis
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Hemolytic-Uremic Syndrome / microbiology
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Humans
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
Substances
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Escherichia coli Proteins