Dose-dependent pharmacokinetics of KR-31378, a new neuroprotective agent for ischaemia-reperfusion damage in dogs

Biopharm Drug Dispos. 2004 Apr;25(3):143-8. doi: 10.1002/bdd.391.

Abstract

Dose-dependent pharmacokinetic parameters of KR-31378, a new neuroprotective agent for ischaemia-reperfusion damage, were evaluated after intravenous and oral administration of the drug at doses of 5, 10 and 25 mg/kg to male beagle dogs. After intravenous administration, the dose-normalized (based on 5 mg/kg) areas under the plasma concentration-time curve from time zero to time infinity (AUC values, 725, 1450 and 2300 micro g min/ml for 5, 10 and 25 mg/kg, respectively) were significantly different among the three dose ranges studied; the value increased more proportionally as the dose increased. This could be due to slower total body clearance (Cl) with increasing doses (6.90, 3.46 and 2.17 ml/min/kg). The slower Cl value with increasing doses may be due to saturable metabolism of KR-31378 in dogs. After oral administration, the dose-normalized (based on 5 mg/kg) AUC values (833, 1450 and 1920 micro g min/ml) at 5 mg/kg were significantly smaller than those at 10 and 25 mg/kg. Note that the AUC values were comparable (not significantly different) between intravenous and oral administration at all doses studied, indicating that the absorption of KR-31378 from the gastrointestinal tract was essentially complete and the first-pass (gastric, intestinal and/or hepatic first-pass) effects were almost negligible in dogs.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Apoptosis / drug effects
  • Area Under Curve
  • Biological Availability
  • Brain Ischemia / complications
  • Dogs
  • Dose-Response Relationship, Drug
  • Guanidines / administration & dosage
  • Guanidines / pharmacokinetics*
  • Infusions, Intravenous
  • Male
  • Neuroprotective Agents / administration & dosage
  • Neuroprotective Agents / pharmacokinetics*
  • Pyrans / administration & dosage
  • Pyrans / pharmacokinetics*
  • Reperfusion Injury / drug therapy
  • Reperfusion Injury / etiology
  • Time Factors

Substances

  • Guanidines
  • N''-cyano-N-(6-amino-3,4-dihydro-3-hydroxy-2-methyl-2-dimethoxymethyl-2H-benzopyran-4-yl)-N'-benzylguanidine
  • Neuroprotective Agents
  • Pyrans