Evaluation of in vivo selective binding of [11C]doxepin to histamine H1 receptors in five animal species

Nucl Med Biol. 2004 May;31(4):493-502. doi: 10.1016/j.nucmedbio.2003.11.005.

Abstract

The specific binding of [(11)C]doxepin, which has been used as a radioligand for mapping histamine H(1) receptors in human brain by positron emission tomography, was evaluated in five animal species. In mice the [(11)C]doxepin uptake was reduced by treatment with cold doxepin and two H(1) receptor antagonists, but not with H(2)/H(3) antagonists. The specific binding evaluated with treatment with (+)-chlorpheniramine (H(1) antagonist) was in the range of 10-30% in mouse, rat, rabbit, and monkey, but was not detected in guinea pig.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / diagnostic imaging*
  • Brain / drug effects
  • Brain / metabolism*
  • Carbon Radioisotopes / pharmacokinetics
  • Doxepin / pharmacokinetics*
  • Guinea Pigs
  • Histamine Agonists / pharmacology
  • Humans
  • Macaca mulatta
  • Male
  • Metabolic Clearance Rate
  • Mice
  • Protein Binding
  • Rabbits
  • Radiopharmaceuticals / pharmacokinetics
  • Rats
  • Rats, Wistar
  • Receptors, Histamine H1 / metabolism*
  • Species Specificity
  • Tissue Distribution
  • Tomography, Emission-Computed / methods

Substances

  • Carbon Radioisotopes
  • Histamine Agonists
  • Radiopharmaceuticals
  • Receptors, Histamine H1
  • Doxepin