Tools for channels: moving towards molecular calculations of gating and permeation in ion channel biophysics

J Mol Graph Model. 2004 May;22(5):359-68. doi: 10.1016/j.jmgm.2003.12.003.

Abstract

Recent X-ray structures of voltage gated potassium channels provide an exciting opportunity to connect molecular structures with measured biological function. Two of the most important connections for these channels are: first, to the molecular basis behind selectivity and the associated free energy profile underlying ionic current flow and, second, to a true molecular understanding of the large-scale conformational transitions that underlie voltage dependent gating. But, existing computational tools need to be further developed to reach these goals. In this contribution to the symposia on sampling methods we outline our dynamic importance sampling method for sampling large-scale conformational transitions as well as our studies with non-equilibrium work events and equilibrium overlap sampling (OS) methods for sampling events related to the calculation of relative free energies.

Publication types

  • Review

MeSH terms

  • Algorithms
  • Animals
  • Biophysical Phenomena
  • Biophysics
  • Computational Biology*
  • Crystallography, X-Ray
  • Humans
  • Ion Channel Gating*
  • Ion Channels / chemistry*
  • Kinetics
  • Models, Molecular*
  • Potassium Channels / chemistry
  • Thermodynamics

Substances

  • Ion Channels
  • Potassium Channels