Construction of a tRNA-embedded-ribozyme trimming plasmid

Biochem Biophys Res Commun. 1992 Aug 14;186(3):1271-9. doi: 10.1016/s0006-291x(05)81543-8.

Abstract

We have combined Cotten and Birnstiel's tRNA-embedded ribozymes and our 5'- and 3'-trimming system. Although the activity of the tRNA-embedded ribozyme was ca. 30% lower than those of naked ribozymes, since the stability of the former in bovine serum was higher than those of naked ribozymes, the tRNA-embedded ribozymes appear useful especially when the 5'- and 3'-trimming units are concatenated in tandem.

MeSH terms

  • Base Sequence
  • DNA / genetics*
  • DNA / metabolism
  • Genetic Techniques
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Oligodeoxyribonucleotides
  • Plasmids*
  • RNA, Catalytic / metabolism*
  • RNA, Transfer / genetics
  • RNA, Transfer / metabolism*
  • Restriction Mapping
  • Templates, Genetic

Substances

  • Oligodeoxyribonucleotides
  • RNA, Catalytic
  • DNA
  • RNA, Transfer