Breakfast cereal fortified with folic acid, vitamin B-6, and vitamin B-12 increases vitamin concentrations and reduces homocysteine concentrations: a randomized trial

Am J Clin Nutr. 2004 May;79(5):805-11. doi: 10.1093/ajcn/79.5.805.

Abstract

Background: High homocysteine and low B vitamin concentrations have been linked to the risk of vascular disease, stroke, and dementia and are relatively common in older adults.

Objective: We assessed the effect of breakfast cereal fortified with folic acid, vitamin B-6, and vitamin B-12 on vitamin and homocysteine status.

Design: A randomized, double-blind trial was conducted in 189 volunteers aged 50-85 y. The subjects had no history of hypertension, anemia, asthma, cancer, or cardiovascular or digestive disease and did not regularly consume multiple or B vitamin supplements or highly fortified breakfast cereal. Subjects were randomly assigned to consume 1 cup (0.24 L) breakfast cereal fortified with 440 microg folic acid, 1.8 mg vitamin B-6, and 4.8 microg vitamin B-12 or placebo cereal for 12 wk. Blood was drawn at 0, 2, 12, and 14 wk. Methionine-loading tests were conducted at baseline and week 14.

Results: Final baseline-adjusted plasma homocysteine concentrations were significantly lower and B vitamin concentrations were significantly higher in the treatment group than in the placebo group (P < 0.001). The percentage of subjects with plasma folate concentrations < 11 nmol/L decreased from 2% to 0%, with vitamin B-12 concentrations < 185 pmol/L from 9% to 3%, with vitamin B-6 concentrations < 20 nmol/L from 6% to 2%, and with homocysteine concentrations > 10.4 micromol/L (women) or > 11.4 micromol/L (men) from 6.4% to 1.6%. The percentage of control subjects with values beyond these cutoff points remained nearly constant or increased.

Conclusions: In this relatively healthy group of volunteers, consumption of 1 cup fortified breakfast cereal daily significantly increased B vitamin and decreased homocysteine concentrations, including post-methionine-load homocysteine concentrations.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Aging / blood
  • Aging / metabolism
  • Cardiovascular Diseases / blood
  • Cardiovascular Diseases / prevention & control
  • Double-Blind Method
  • Edible Grain*
  • Female
  • Folic Acid / administration & dosage*
  • Folic Acid / metabolism
  • Food, Fortified*
  • Homocysteine / blood*
  • Homocysteine / drug effects
  • Humans
  • Male
  • Methionine / metabolism
  • Middle Aged
  • Vitamin B 12 / administration & dosage*
  • Vitamin B 12 / metabolism
  • Vitamin B 6 / administration & dosage*
  • Vitamin B 6 / metabolism

Substances

  • Homocysteine
  • Vitamin B 6
  • Folic Acid
  • Methionine
  • Vitamin B 12