Detection of polymorphisms of human DNA after polymerase chain reaction by miniaturized SDS-PAGE

F Barros; M V Lareu; A Carracedo

doi:10.1016/0379-0738(92)90091-a

Detection of polymorphisms of human DNA after polymerase chain reaction by miniaturized SDS-PAGE

Forensic Sci Int. 1992 Jul;55(1):27-36. doi: 10.1016/0379-0738(92)90091-a.

Authors

F Barros¹, M V Lareu, A Carracedo

Affiliation

¹ Institute of Legal Medicine, Faculty of Medicine, University of Santiago de Compostela, Spain.

PMID: 1511936
DOI: 10.1016/0379-0738(92)90091-a

Abstract

PCR followed by SDS-PAGE in miniaturized non-denaturing gels permits in some cases the identification of single base pair substitutions in small DNA fragments and therefore, the study of human DNA polymorphisms. The usefulness of the system in forensic science is investigated by typing the HLA-DQA1 locus and the VNTR recognized with the probe pMCT118 (locus D1S80) and it shows to be advantageous over previously published methods for typing the MCT118 system, whereas in HLA-DQA1 typing for forensic casework, both dot-blot with ASO probes and this method could be complementary.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Blood Stains
DNA / analysis*
Electrophoresis, Polyacrylamide Gel / methods*
Forensic Medicine / methods*
HLA-DQ Antigens / genetics
HLA-DQ alpha-Chains
Hair / chemistry
Humans
Molecular Sequence Data
Nucleic Acid Amplification Techniques
Phenotype
Polymerase Chain Reaction*

Substances

HLA-DQ Antigens
HLA-DQ alpha-Chains
HLA-DQA1 antigen
DNA