Abstract
Thirty-eight avian and swine French isolates of Campylobacter coli were studied for their mechanisms of co-resistance to fluoroquinolones and erythromycin. A Thr86Ile modification of GyrA, responsible for fluoroquinolone resistance, was found in all the strains. Two different levels of resistance to erythromycin (MIC of 8-16 or >/=256 mg/l) were observed. A A2075G mutation in the 23S rRNA genes was found only in the highly-resistant strains. Phe-Arg-beta-naphthylamide, an efflux pump inhibitor, potentiated erythromycin in all the strains examined but restored susceptibility only in the strains with a low-level of resistance. This suggests the involvement of efflux in intrinsic and in acquired low-level of resistance to erythromycin in C. coli.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Substitution
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Animals
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Bacterial Proteins / genetics
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Biological Transport / drug effects
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Campylobacter coli / drug effects*
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Campylobacter coli / genetics*
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Campylobacter coli / isolation & purification
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DNA Fingerprinting
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DNA Gyrase / genetics
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DNA, Bacterial / analysis
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DNA, Bacterial / isolation & purification
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Dipeptides / pharmacology
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Drug Resistance, Multiple, Bacterial / genetics*
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Drug Synergism
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Erythromycin / metabolism
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Erythromycin / pharmacology*
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Fluoroquinolones / metabolism
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Fluoroquinolones / pharmacology*
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Food Microbiology
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France
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Genes, Bacterial
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Genes, rRNA
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Membrane Transport Modulators
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Membrane Transport Proteins / antagonists & inhibitors
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Microbial Sensitivity Tests
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Mutation
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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Poultry / microbiology*
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RNA, Ribosomal, 23S / genetics
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Swine / microbiology*
Substances
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Bacterial Proteins
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DNA, Bacterial
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Dipeptides
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Fluoroquinolones
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Membrane Transport Modulators
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Membrane Transport Proteins
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RNA, Ribosomal, 23S
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phenylalanylarginine-naphthylamide
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Erythromycin
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DNA Gyrase