Abstract
Human herpesvirus-8 (HHV-8/KSHV) is etiologically associated with Kaposi's sarcoma (KS) and other tumors. Constitutive activation of the mitogen-activated protein kinase (MAPK) signaling pathway has been associated with a variety of tumors, including AIDS-related KS. The oncoprotein Raf is situated at a pivotal position in regulating the MAPK pathway. Hence, we analysed the effect of oncoprotein Raf on HHV-8 infectious entry into target cells. Here we report Raf expression to significantly enhance HHV-8 infection of target cells. These findings implicate a role for Raf not only in the infectious entry of HHV-8 but also in modulating KS pathogenesis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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AIDS-Related Opportunistic Infections / virology
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Blotting, Western
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Butadienes / pharmacology
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Cell Line
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Enzyme Activation
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Enzyme Inhibitors / pharmacology
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Epidermal Growth Factor / pharmacology
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Estradiol / pharmacology
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Green Fluorescent Proteins
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Heparin-binding EGF-like Growth Factor
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Herpesviridae Infections*
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Herpesvirus 8, Human / metabolism*
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Herpesvirus 8, Human / pathogenicity*
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Humans
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Intercellular Signaling Peptides and Proteins
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Luminescent Proteins / metabolism
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Mitogen-Activated Protein Kinase Kinases / drug effects
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Mitogen-Activated Protein Kinase Kinases / metabolism
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Mitogen-Activated Protein Kinases / drug effects
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Mitogen-Activated Protein Kinases / metabolism
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Nitriles / pharmacology
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Proto-Oncogene Proteins c-raf / metabolism*
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Receptors, Estrogen / chemistry
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / drug effects
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Recombinant Fusion Proteins / metabolism
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Sarcoma, Kaposi / etiology
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Sarcoma, Kaposi / virology
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Signal Transduction
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Vascular Endothelial Growth Factor A / pharmacology
Substances
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Butadienes
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Enzyme Inhibitors
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HBEGF protein, human
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Heparin-binding EGF-like Growth Factor
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Intercellular Signaling Peptides and Proteins
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Luminescent Proteins
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Nitriles
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Receptors, Estrogen
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Recombinant Fusion Proteins
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U 0126
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Vascular Endothelial Growth Factor A
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Green Fluorescent Proteins
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Estradiol
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Epidermal Growth Factor
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Proto-Oncogene Proteins c-raf
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Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinase Kinases