Absence of KIF1B mutation in a large Turkish CMT2A family suggests involvement of a second gene

Neurology. 2004 May 11;62(9):1522-5. doi: 10.1212/01.wnl.0000123253.57555.3a.

Abstract

Background: Charcot-Marie-Tooth disease type 2A (CMT2A) was assigned to a 19.3-cM region on chromosome 1p35-36. A missense mutation in the kinesin family member 1B gene (KIF1B) was reported in a single CMT2A family.

Objective: To report the clinical and genetic data of a Turkish family with CMT2A.

Methods: Linkage to CMT2 loci was investigated in the family. Haplotype analysis of the CMT2A region was completed using additional single-nucleotide polymorphism and short tandem repeat markers. The KIF1B gene was sequenced on genomic DNA and cDNA in two patients.

Results: A recombination event narrowed the CMT2A locus to a 9.3-cM region flanked by D1S160 and D1S434. No mutation in KIF1B was found.

Conclusion: The exclusion of KIF1B gene mutations in this family suggests the involvement of another CMT2A gene in the linked region.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Adult
  • Carrier Proteins / genetics*
  • Cell Cycle Proteins / genetics*
  • Charcot-Marie-Tooth Disease / diagnosis
  • Charcot-Marie-Tooth Disease / ethnology
  • Charcot-Marie-Tooth Disease / genetics*
  • Child
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1 / genetics
  • Ethnicity / genetics*
  • Family / ethnology
  • Female
  • Genetic Linkage / genetics
  • Genetic Markers / genetics
  • Humans
  • Kinesins / genetics*
  • Lod Score
  • Male
  • Middle Aged
  • Mutation / genetics*
  • Mutation, Missense / genetics
  • Nerve Tissue Proteins / genetics*
  • Neural Conduction / physiology
  • Nuclear Proteins
  • Pedigree
  • Phenotype
  • Polymorphism, Single Nucleotide / genetics
  • Tandem Repeat Sequences / genetics
  • Turkey / ethnology

Substances

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • Cell Cycle Proteins
  • Genetic Markers
  • KIF1B protein, human
  • MAD2L1BP protein, human
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • Kinesins