Molecular analysis of a gene from Bacteroides fragilis involved in metronidazole resistance in Escherichia coli

G U Wehnert; V R Abratt; D R Woods

doi:10.1016/0147-619x(92)90027-8

Molecular analysis of a gene from Bacteroides fragilis involved in metronidazole resistance in Escherichia coli

Plasmid. 1992 May;27(3):242-5. doi: 10.1016/0147-619x(92)90027-8.

Authors

G U Wehnert¹, V R Abratt, D R Woods

Affiliation

¹ Department of Microbiology, University of Cape Town, Rondebosch, South Africa.

PMID: 1513881
DOI: 10.1016/0147-619x(92)90027-8

Abstract

The region of Bacteroides fragilis DNA on the recombinant plasmid pMT100 responsible for conferring metronidazole resistance in Escherichia coli strains was characterized. An open reading frame (ORF1) of 195 bp encoded a protein of 64 amino acids with a predicted M(r) of 7.3 kDa. Deletion analysis indicated that ORF1 conferred the metronidazole resistance phenotype and encoded a protein with an apparent M(r) of approximately 8-10 kDa.

MeSH terms

Amino Acid Sequence
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Bacteroides fragilis / drug effects
Bacteroides fragilis / genetics*
Base Sequence
Drug Resistance, Microbial / genetics
Escherichia coli / drug effects*
Escherichia coli / genetics
Genes, Bacterial
Metronidazole / pharmacology*
Molecular Sequence Data
Open Reading Frames
Recombinant Fusion Proteins / metabolism

Substances

Bacterial Proteins
Recombinant Fusion Proteins
Metronidazole

Associated data

GENBANK/M76551