Abstract
In order to develop a specific tool differentiating the African field strains of Mycoplasma mycoides subsp. mycoides SC from other potentially less virulent strains, including the vaccine strains, we have developed a PCR followed by a restriction enzyme analysis (PCR-REA). This approach also differentiates the African field strains from the Australian strains and the type strain PG1. The genomic marker detected by the PCR-REA is based on a single nucleotide change in the bgl gene that codes for 6-phospho-beta-glucosidase (Bgl), an enzyme that is involved in sugar metabolism.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Africa
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Animals
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Base Sequence
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Cattle
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Cluster Analysis
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Genetic Markers
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Glucosidases / chemistry
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Glucosidases / genetics
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Mycoplasma mycoides / classification
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Mycoplasma mycoides / genetics*
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Mycoplasma mycoides / pathogenicity
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Polymerase Chain Reaction / veterinary
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Virulence
Substances
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DNA, Bacterial
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Genetic Markers
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endodeoxyribonuclease SpeI
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Deoxyribonucleases, Type II Site-Specific
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Glucosidases
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6-phospho-beta-glucosidase