Aim: To explore the expression and regulation of a novel soluble adhesive molecule sCD226 on HUVECs.
Methods: The contents of sCD226 and NO in culture supernatant of LPS activated human umbilical vein endothelial cells (HUVECs) were detected by ELISA and Greiss method respectively, and linear correlativity of contents between sCD226 and NO was analysed. The influence of anti-TNF-alpha mAb and iNOS inhibitor on LPS-induced sCD226 production was observed by ELISA.
Results: (1)LPS might markedly stimulate sCD226 production from HUVECs in a dose- and time-dependent manner. The amount of sCD226 came to 188.5 microg/L when endothelial cells were stimulated by 100 mg/L LPS for 3 days. (2)The production of NO and sCD226 showed linear correlation when HUVECs were stimulated by 100 mg/L LPS.(3)LPS-induced sCD226 production in HUVECs was inhibited significantly by anti-TNF-alpha mAb and iNOS inhibitor.
Conclusion: LPS-activated-HUVECs can secret sCD226 and the generation of sCD226 is closely correlated with the production of NO and TNF-alpha.