Aim: To prepare monoclonal antibodies against human TACI and study the distribution of TACI on peripheral blood nuclear cells(PBMCs).
Methods: Murine mAbs were prepared by hybridoma technique. The distribution of TACI on PBMCs were identified by immunofluorescence staining, FCM analysis as well as immunohistochemical staining.
Results: Two hybridoma cell lines secreting mAbs against TACI were obtained. The titers of ascitic mAbs was 10(-7) and their Ig subclasses were IgG1. Both could recognize natural TACI on T cells. In the PHA activated models, TACI was expressed mainly on activated CD4(+) T cells.
Conclusion: Two mAbs can recognize natural TACI, which will be of greater value in research on the structure and functions of TACI.