Abstract
A clinical strain of Escherichia coli isolated from pleural liquid with high levels of resistance to cefotaxime, ceftazidime, and aztreonam harbors a novel CTX-M gene (bla(CTX-M-32)) whose amino acid sequence differs from that of CTX-M-1 by a single Asp240-Gly substitution. Moreover, by site-directed mutagenesis we demonstrated that this replacement is a key event in ceftazidime hydrolysis
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acid Substitution
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Ceftazidime / pharmacology*
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Cephalosporin Resistance / genetics*
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Cephalosporins / pharmacology*
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DNA, Bacterial / genetics
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / drug effects*
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Escherichia coli / genetics*
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Escherichia coli Infections / microbiology
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Isoelectric Focusing
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Kinetics
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Molecular Sequence Data
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Mutagenesis, Site-Directed / genetics
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Plasmids / genetics
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Pleura / microbiology
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beta-Lactamases / genetics*
Substances
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Cephalosporins
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DNA, Bacterial
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Ceftazidime
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beta-lactamase TEM-3
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beta-Lactamases
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beta-lactamase CTX-M-32, E coli