Abstract
Major surface protein 2 (MSP2) and MSP3 of the persistent bovine ehrlichial pathogen Anaplasma marginale are immunodominant proteins that undergo antigenic variation. The recently completed sequence of MSP3 revealed blocks of amino acids in the N and C termini that are conserved with MSP2. This study tested the hypothesis that CD4+ T cells specific for MSP2 recognize naturally processed epitopes conserved in MSP3. At least one epitope in the N terminus and two in the C terminus of MSP2 were also processed from MSP3 and presented to CD4+ T lymphocytes from MSP2-immunized cattle. This T-lymphocyte response to conserved and partially conserved epitopes may contribute to the immunodominance of MSP2 and MSP3.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Anaplasma / immunology*
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Anaplasmosis / prevention & control
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Animals
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Antigens, Bacterial / chemistry
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Antigens, Bacterial / immunology*
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Bacterial Outer Membrane Proteins / chemistry
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Bacterial Outer Membrane Proteins / immunology*
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Bacterial Proteins / chemistry
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Bacterial Proteins / immunology*
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Bacterial Vaccines / immunology*
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CD4-Positive T-Lymphocytes / immunology*
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Cattle
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Cattle Diseases / prevention & control
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Cells, Cultured
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Conserved Sequence
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Epitope Mapping
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Epitopes, T-Lymphocyte / immunology*
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Immunization
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Immunodominant Epitopes / immunology
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Molecular Sequence Data
Substances
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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Bacterial Vaccines
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Epitopes, T-Lymphocyte
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Immunodominant Epitopes
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major surface protein 3, Anaplasma marginale
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msp2 protein, Anaplasma marginale