The novel Myb transcription factor LCR1 regulates the CO2-responsive gene Cah1, encoding a periplasmic carbonic anhydrase in Chlamydomonas reinhardtii

Satoshi Yoshioka; Fumiya Taniguchi; Kenji Miura; Takeshi Inoue; Takashi Yamano; Hideya Fukuzawa

doi:10.1105/tpc.021162

The novel Myb transcription factor LCR1 regulates the CO2-responsive gene Cah1, encoding a periplasmic carbonic anhydrase in Chlamydomonas reinhardtii

Plant Cell. 2004 Jun;16(6):1466-77. doi: 10.1105/tpc.021162. Epub 2004 May 21.

Authors

Satoshi Yoshioka¹, Fumiya Taniguchi, Kenji Miura, Takeshi Inoue, Takashi Yamano, Hideya Fukuzawa

Affiliation

¹ Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.

Abstract

Chlamydomonas reinhardtii acclimates to CO2-limiting stress by inducing a set of genes for a carbon-concentrating mechanism (CCM). This set includes the gene Cah1, which encodes a periplasmic carbonic anhydrase. Although physiological aspects of CO2response have been extensively studied, regulatory components, such as transcription factors involved in the acclimation, have not been well described in eukaryotic microalgae. Using an arylsulfatase gene driven by the Cah1 promoter, a regulatory mutant of Cah1 was isolated and named lcr1 (for low-CO2 stress response). The photosynthetic affinity for inorganic carbon of lcr1 was reduced compared with that of wild-type cells. Expression of three low-CO2-inducible genes, Cah1, Lci1, and Lci6, were regulated by LCR1 as shown by cDNA array and RNA gel blot analyses. The Lcr1 gene encodes a protein of 602 amino acids containing a single Myb domain, which binds to the Cah1-promoter region. Expression of Lcr1 was induced by lowering CO2 levels and controlled by the regulatory factor CCM1. These results suggest that LCR1 transmits the low CO2 signal to at least three CO2-responsive genes and then fully induces CCM.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing
Algal Proteins / genetics
Algal Proteins / metabolism
Amino Acid Sequence
Animals
Carbon Dioxide / pharmacology*
Carbonic Anhydrases / genetics*
Carbonic Anhydrases / metabolism
Chlamydomonas reinhardtii / drug effects
Chlamydomonas reinhardtii / enzymology
Chlamydomonas reinhardtii / genetics*
Chlamydomonas reinhardtii / metabolism
DNA / genetics
DNA / metabolism
Gene Expression Regulation, Enzymologic / drug effects*
Genetic Complementation Test
Molecular Sequence Data
Mutation / genetics
Oligonucleotide Array Sequence Analysis
Periplasm / enzymology*
Plant Proteins
Promoter Regions, Genetic / genetics
Protein Structure, Tertiary
Proto-Oncogene Proteins c-myb / chemistry
Proto-Oncogene Proteins c-myb / genetics
Proto-Oncogene Proteins c-myb / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Substrate Specificity
Trans-Activators / genetics
Trans-Activators / metabolism
Transcription Factors / chemistry
Transcription Factors / metabolism*

Substances

Adaptor Proteins, Signal Transducing
Algal Proteins
Plant Proteins
Proto-Oncogene Proteins c-myb
RNA, Messenger
Trans-Activators
Transcription Factors
ccm1 protein, Chlamydomonas reinhardtii
Carbon Dioxide
DNA
Carbonic Anhydrases

Associated data

GENBANK/AB168090
GENBANK/AB168091