The proximal 2-kb of the Hoxa3 promoter directs gene expression in distinct branchial compartments and cranial ganglia

Nata Y S-G Diman; Ethel Chauvier; Nathalie Pacico; Jacques J Picard; René Rezsohazy

doi:10.1016/j.devbrainres.2004.03.013

The proximal 2-kb of the Hoxa3 promoter directs gene expression in distinct branchial compartments and cranial ganglia

Brain Res Dev Brain Res. 2004 Jun 21;150(2):211-3. doi: 10.1016/j.devbrainres.2004.03.013.

Authors

Nata Y S-G Diman¹, Ethel Chauvier, Nathalie Pacico, Jacques J Picard, René Rezsohazy

Affiliation

¹ Unit of Developmental Genetics, Université Catholique de Louvain, 73 (box 82) Avenue Mounier, Brussels 1200, Belgium.

PMID: 15158084
DOI: 10.1016/j.devbrainres.2004.03.013

Abstract

Developing structures such as hindbrain, neural crest cells or spinal cord express Hoxa3. Here, we have investigated the regulatory role of a 2-kb fragment spanning the proximal promoter of Hoxa3 by a reporter-based approach in mice. We show that this fragment promotes reporter activity in ganglionic and branchial compartments known to express Hoxa3 but for which no cis-regulatory elements have been identified so far. We also show that the 2-kb promoter fragment is active in rhombomere 4 and in the ganglion of the cranial nerve complex VII/VIII that are devoid of Hoxa3 expression.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Branchial Region / embryology
Branchial Region / metabolism*
Cranial Nerves / embryology
Cranial Nerves / metabolism*
Embryo, Mammalian
Embryonic Induction
Ganglia, Autonomic / embryology
Ganglia, Autonomic / metabolism*
Gene Expression Regulation, Developmental*
Homeodomain Proteins / genetics
Homeodomain Proteins / metabolism*
Homeodomain Proteins / physiology
Mice
Mice, Transgenic
Molecular Sequence Data
Promoter Regions, Genetic*
Rhombencephalon / embryology
Rhombencephalon / metabolism*

Substances

Homeodomain Proteins
Hoxa3 protein, mouse