A cocaine analog and a GBR analog label the same protein in rat striatal membranes

Brain Res. 1992 Mar 27;576(1):173-4. doi: 10.1016/0006-8993(92)90627-l.

Abstract

Because some evidence suggests that cocaine and GBR12935 bind to different sites, we utilized photoaffinity probes from both classes of compounds to see if they label the same protein. [125I]RTI-82 a cocaine analog, and [125I]DEEP, a GBR analog, labeled protein(s) showing the same molecular weight, a similar pharmacological profile and a similar sensitivity to neuraminidase.

MeSH terms

  • Animals
  • Azides / metabolism
  • Azides / pharmacology*
  • Binding Sites
  • Carrier Proteins / isolation & purification
  • Carrier Proteins / metabolism*
  • Cell Membrane / metabolism
  • Cocaine / analogs & derivatives*
  • Cocaine / metabolism
  • Cocaine / pharmacology
  • Corpus Striatum / metabolism*
  • Dopamine / metabolism
  • Dopamine Plasma Membrane Transport Proteins
  • Electrophoresis, Polyacrylamide Gel
  • Iodine Radioisotopes
  • Ligands
  • Membrane Glycoproteins / isolation & purification
  • Membrane Glycoproteins / metabolism
  • Membrane Transport Proteins*
  • Nerve Tissue Proteins*
  • Piperazines / metabolism*
  • Rats

Substances

  • Azides
  • Carrier Proteins
  • Dopamine Plasma Membrane Transport Proteins
  • Iodine Radioisotopes
  • Ligands
  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • Nerve Tissue Proteins
  • Piperazines
  • RTI 82
  • 1-(2 (diphenylmethoxy)ethyl)-4-(3-phenylpropyl)piperazine
  • Cocaine
  • Dopamine