[Expressions of c-fos in different brain regions after microinjection of 5-HT3 receptors agonist in hippocampus]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2003 Sep;19(5):454-5, 458.
[Article in Chinese]

Abstract

Aim: To explore the neuronal circuit and possible pathway related to the 5-HT3 receptors in hippocampus neuroimmunomodulation.

Methods: SABC immunocytochemical staining was used to detect the c-fos expression.

Results: A large number of FOS-positive neurons expression were found in the hippocampus and brain cortex at 1 hour after microinjection of 5-HT3 receptor agonist (I-PBG) in the hippocampus, but as time went on, the number of FOS positive-neurons gradually decrease. A small number of FOS positive-neurons were found only in the hypothalamus and gray matter around acqueduct of midbrain, respectively at 8 and 16 hs after injection of 1-PBG. The expression of c-fos induced by 1-PBG could be blocked by pre-microinjection of tropisetron.

Conclusion: Brain cortex-hippocampus hypothalamus-gray matter around acqueduct of midbrain forma neuronal network, which may be related to immunomodulation.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Biguanides / pharmacology*
  • Brain Chemistry / drug effects*
  • Female
  • Hippocampus / drug effects*
  • Immunohistochemistry
  • Male
  • Microinjections
  • Proto-Oncogene Proteins c-fos / analysis*
  • Rats
  • Rats, Sprague-Dawley
  • Serotonin Receptor Agonists / pharmacology*

Substances

  • Biguanides
  • Proto-Oncogene Proteins c-fos
  • Serotonin Receptor Agonists
  • phenyl biguanide