Toxoplasma gondii: generation of novel truncation mutations in the linker domain of dihydrofolate reductase-thymidylate synthase

Exp Parasitol. 2004 Mar-Apr;106(3-4):179-82. doi: 10.1016/j.exppara.2004.03.002.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antiprotozoal Agents / pharmacology
  • Blotting, Western
  • Drug Resistance / genetics
  • Humans
  • Models, Genetic
  • Molecular Sequence Data
  • Multienzyme Complexes / chemistry
  • Multienzyme Complexes / genetics*
  • Mutagenesis, Insertional
  • Mutation*
  • Point Mutation
  • Polymerase Chain Reaction
  • Pyrimethamine / pharmacology
  • Restriction Mapping
  • Sequence Alignment
  • Tetrahydrofolate Dehydrogenase / chemistry
  • Tetrahydrofolate Dehydrogenase / genetics*
  • Thymidylate Synthase / chemistry
  • Thymidylate Synthase / genetics*
  • Toxoplasma / drug effects
  • Toxoplasma / enzymology
  • Toxoplasma / genetics*
  • Transfection

Substances

  • Antiprotozoal Agents
  • Multienzyme Complexes
  • thymidylate synthase-dihydrofolate reductase
  • Tetrahydrofolate Dehydrogenase
  • Thymidylate Synthase
  • Pyrimethamine