A powerful antiradiation compound revealed by a new high-throughput screening method

Chembiochem. 2004 Jun 7;5(6):832-40. doi: 10.1002/cbic.200300787.

Abstract

We present a new high-throughput screening method for the selection of powerful water-soluble antiradiation compounds. This method, which uses conventional immunoassay techniques, allowed the capacity of a given compound to protect thymidine from irradiation to be evaluated. By applying this assay to an antioxidant library, we showed for the first time that norbadione A, a well-known mushroom pigment, has pronounced atypical antiradiation properties.

Publication types

  • Comparative Study

MeSH terms

  • 4-Butyrolactone / analogs & derivatives*
  • 4-Butyrolactone / chemistry
  • 4-Butyrolactone / pharmacology*
  • Agaricales / chemistry
  • Antioxidants / chemistry
  • Antioxidants / classification
  • Antioxidants / pharmacology
  • Biological Factors / chemistry
  • Biological Factors / pharmacology
  • DNA / radiation effects
  • Dose-Response Relationship, Drug
  • Drug Evaluation, Preclinical / methods*
  • Evaluation Studies as Topic
  • Immunoassay / methods*
  • Phenylacetates / chemistry
  • Phenylacetates / pharmacology*
  • Plant Extracts / chemistry
  • Radiation-Protective Agents / chemistry
  • Radiation-Protective Agents / pharmacology*
  • Solubility
  • Thymidine / chemistry*
  • Thymidine / radiation effects
  • Time Factors

Substances

  • Antioxidants
  • Biological Factors
  • Phenylacetates
  • Plant Extracts
  • Radiation-Protective Agents
  • DNA
  • norbadione A
  • 4-Butyrolactone
  • Thymidine