Objective: To study the change of beta 1-integrin in neonatal rats with ischemic renal injury.
Methods: Twenty-four neonatal rats weighing from 30 to 40 g were chosen. Anesthesia was carried out by peritoneal injection of nembutal-sodium solution (4 mg/100 g). Renal ischemic injury model was induced by clamping the left renal pedicle for different periods of time. The kidneys of normal neonatal rats were functioned as the control group. Immunofluorescence technique was used to detect the location of beta 1-integrin in kidney, especially in cortex.
Results: In control group, beta 1-integrin subunit was found to be located mainly in the basal membrane of tubular epithelial cell. After 10 minutes of ischemia, immunostaining highlighted the broken basement membranes of the experiment groups. The longer the ischemia continued, the more the broken points we observed. There were significant differences between the experiment groups and the control group. Yet, no significant difference in the IOD value of beta 1-integrin was seen between the 10-min-ischemia and 30-min-ischemia groups.
Conclusion: Under physiological condition, beta 1-integrin is distributed over the basal membrane of the tubular epithelial cell of neonatal rat. Ischemic injury can cause the changes of beta 1-integrin which may be the reason of exfoliation and depolarization of tubular epithelia cell.