Abstract
Giardia cysts in 131 raw wastewater samples from Milwaukee, Wis., were genotyped by sequence analysis of the triosephosphate isomerase gene which showed the presence of two distinct genotypes (assemblages A and B) of Giardia duodenalis. Of the 131 samples, 111 belonged to assemblage A, and the remaining samples belonged to assemblage B. A high degree of genetic polymorphism was evident within the assemblage B cluster, with 10 distinct subgenotypes identified, eight of which have not been reported before.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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DNA, Protozoan / analysis
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Genetic Variation
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Genotype
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Giardia / classification*
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Giardia / genetics*
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Humans
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Molecular Sequence Data
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Polymerase Chain Reaction
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Sewage / microbiology*
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Triose-Phosphate Isomerase / genetics
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Waste Disposal, Fluid*
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Water Microbiology*
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Wisconsin
Substances
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DNA, Protozoan
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Sewage
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Triose-Phosphate Isomerase
Associated data
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GENBANK/AY368157
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GENBANK/AY368158
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GENBANK/AY368159
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GENBANK/AY368160
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GENBANK/AY368161
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GENBANK/AY368162
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GENBANK/AY368163
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GENBANK/AY368164
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GENBANK/AY368165
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GENBANK/AY368166
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GENBANK/AY368167
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GENBANK/AY368168
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GENBANK/AY368169
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GENBANK/AY368170
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GENBANK/AY368171