Nitro and amino substitution within the A-ring of 5H-8,9-dimethoxy-5-(2-N,N-dimethylaminoethyl)dibenzo[c,h][1,6]naphthyridin-6-ones: influence on topoisomerase I-targeting activity and cytotoxicity

Bioorg Med Chem. 2004 Jul 1;12(13):3731-42. doi: 10.1016/j.bmc.2004.03.076.

Abstract

Recently, 5H-8,9-dimethoxy-5-(2-N,N-dimethylaminoethyl)-2,3-methylenedioxydibenzo[c,h][1,6]naphthyridin-6-one, 1, was identified as a TOP1-targeting agent with pronounced antitumor activity. In the present study, the effect on activity of substituting a single nitro or amino group in the A-ring in lieu of the methylenedioxy moiety of 1 was evaluated. The presence of either a nitro or amino substituent at the 4-position had a pronounced adverse affect on both TOP1-targeting activity and cytotoxicity. To a lesser extent, derivatives with a nitro or amino substituent at the 1-position were also less active than 1. Replacement of the methylenedioxy moiety of 1 with either a nitro or amino substituent at either the 2- and 3-position did result in analogues with potent TOP1-targeting activity and cytotoxicity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amination
  • Antineoplastic Agents / chemical synthesis
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology
  • Antineoplastic Agents / toxicity
  • Cell Line, Tumor
  • DNA / metabolism
  • DNA Topoisomerases, Type I / metabolism*
  • Enzyme Inhibitors / chemical synthesis
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology
  • Enzyme Inhibitors / toxicity
  • Humans
  • Inhibitory Concentration 50
  • Molecular Conformation
  • Molecular Structure
  • Naphthyridines / chemical synthesis
  • Naphthyridines / chemistry*
  • Naphthyridines / pharmacology*
  • Naphthyridines / toxicity
  • Nitrogen / chemistry*
  • Static Electricity
  • Topoisomerase I Inhibitors*

Substances

  • Antineoplastic Agents
  • Enzyme Inhibitors
  • Naphthyridines
  • Topoisomerase I Inhibitors
  • benzonaphthyridone
  • DNA
  • DNA Topoisomerases, Type I
  • Nitrogen