Abstract
We used enzyme-linked immunoassay (ELISA), immunohistochemistry (IHC), and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to determine whether interleukin (IL)-10 protein is changed after unilateral crush or chronic constriction injury (CCI) of mouse or rat sciatic nerve and whether IL-10 protein and mRNA are differentially regulated. In the mouse sciatic nerve, IL-10 protein declined rapidly to 10-20% of baseline early after crush or CCI, while the IL10 mRNA was up-regulated with a maximum on Days 1 and 3. In the rat sciatic nerve, IL-10 protein was significantly reduced on Day 3 after CCI, and IL-10 mRNA was up-regulated in both models. These results suggest that changes of the local cytokine network during wallerian degeneration include an early deficiency of the antiinflammatory cytokine IL-10 despite up-regulation at the mRNA level.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Axons / metabolism
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Axons / pathology
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Chronic Disease
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Disease Models, Animal
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Disease Progression
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Down-Regulation / genetics
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Female
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Inflammation / genetics
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Inflammation / immunology
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Inflammation / metabolism
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Interleukin-10 / deficiency
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Interleukin-10 / genetics
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Interleukin-10 / metabolism*
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Ligation
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Mice
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Mice, Inbred C57BL
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Nerve Crush
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Peripheral Nerves / cytology
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Peripheral Nerves / metabolism*
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Peripheral Nervous System Diseases / genetics
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Peripheral Nervous System Diseases / immunology
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Peripheral Nervous System Diseases / metabolism*
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Reaction Time / genetics
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Sciatic Nerve / metabolism*
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Sciatic Nerve / pathology
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Sciatic Nerve / physiopathology
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Sciatic Neuropathy / genetics
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Sciatic Neuropathy / immunology
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Sciatic Neuropathy / metabolism*
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Up-Regulation / genetics
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Wallerian Degeneration / genetics
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Wallerian Degeneration / immunology
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Wallerian Degeneration / metabolism*
Substances
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RNA, Messenger
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Interleukin-10