Abstract
GABA uptake as well as the distribution of GAT-1, GAT-2 and GAT-3 were investigated in bovine brain membrane fractions. GABA uptake was characterised by kinetic constants and IC50-values for a series of known inhibitors in subcellular fractions of frontal cortex and brainstem obtained by subsequent centrifugations on sucrose gradients. Additionally, the immunoreactivity for rGAT-1, rGAT-2 and rGAT-3 antibodies was studied in these fractions. The pharmacological profile for GABA uptake inhibition as well as results from immunoblotting indicated that GABA uptake in a selected subcellular fraction of frontal cortex (P2B) is almost exclusively due to GAT-1 whereas GABA uptake performed with a selected subcellular fraction of brainstem (P2A) in the presence of NNC 711 is mainly attributable to GAT-3.
Copyright 2004 Elsevier Ireland Ltd.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western / methods
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Brain Stem / cytology
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Brain Stem / drug effects
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Brain Stem / metabolism*
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Carrier Proteins / metabolism
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Cattle
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Frontal Lobe / cytology
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Frontal Lobe / drug effects
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Frontal Lobe / metabolism*
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GABA Plasma Membrane Transport Proteins
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Inhibitory Concentration 50
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Kidney / metabolism
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Membrane Proteins / metabolism
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Membrane Transport Proteins*
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Neurotransmitter Uptake Inhibitors / pharmacology
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Nipecotic Acids / pharmacology
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Oximes / pharmacology
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Sodium
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Subcellular Fractions / drug effects
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Subcellular Fractions / metabolism
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gamma-Aminobutyric Acid / metabolism*
Substances
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Carrier Proteins
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GABA Plasma Membrane Transport Proteins
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Membrane Proteins
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Membrane Transport Proteins
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Neurotransmitter Uptake Inhibitors
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Nipecotic Acids
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Oximes
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NNC 711
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gamma-Aminobutyric Acid
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Sodium