Abstract
RNA interference has been applied for the prevention of virus infections in mammalian cells but has not succeeded in eliminating infections from already infected cells. We now show that the transfection of JC virus-infected SVG-A human glial cells with small interfering RNAs that target late viral proteins, including agnoprotein and VP1, results in a marked inhibition both of viral protein expression and of virus production. RNA interference directed against JC virus genes may thus provide a basis for the development of new strategies to control infections with this polyomavirus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Capsid Proteins / genetics
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Capsid Proteins / metabolism
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Cell Line, Transformed
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Gene Expression Regulation, Viral*
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Humans
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JC Virus / pathogenicity*
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JC Virus / physiology*
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Neuroglia / virology
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RNA Interference*
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RNA, Small Interfering / genetics
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Transfection
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Viral Proteins / genetics
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Viral Proteins / metabolism
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Viral Regulatory and Accessory Proteins
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Virus Replication
Substances
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Capsid Proteins
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RNA, Small Interfering
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VP1 protein, polyomavirus
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Viral Proteins
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Viral Regulatory and Accessory Proteins
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agnoprotein, polyomavirus