Abstract
Ipaf has been associated with apoptosis, cytokine processing, and nuclear factor-kappaB activation. Here, we describe that Ipaf is highly expressed in myelomonocytic cells and that the mRNA levels of Ipaf progressively increase during differentiation of CD34(+) progenitors to granulocytes and monocytes. Additionally, treatment with tumor necrosis factor-alpha and exposure to UV radiation induced the transcriptional activation of Ipaf in human leukemia HL-60 cells. Thus, Ipaf may contribute to modulate the response of myeloid cells to genotoxic and pro-inflammatory stimuli.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis Regulatory Proteins
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Base Sequence
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CARD Signaling Adaptor Proteins
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Calcium-Binding Proteins / genetics
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Calcium-Binding Proteins / physiology*
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Cell Line, Tumor
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DNA Primers
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Electrophoretic Mobility Shift Assay
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Humans
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Leukemia / pathology
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Leukemia / physiopathology*
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Tumor Necrosis Factor-alpha / pharmacology*
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Ultraviolet Rays
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Up-Regulation / drug effects*
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Up-Regulation / radiation effects
Substances
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Apoptosis Regulatory Proteins
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CARD Signaling Adaptor Proteins
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Calcium-Binding Proteins
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DNA Primers
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NLRC4 protein, human
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Tumor Necrosis Factor-alpha