Synthesis of oligonucleotide-peptide and oligonucleotide-protein conjugates

Methods Mol Biol. 2004:283:197-206. doi: 10.1385/1-59259-813-7:197.

Abstract

The conjugation of macromolecules offers a rapid and versatile route to improved function. Here, the methods for obtaining disulfide-linked oligonucleotide-peptide and oligonucleotide-protein conjugates are described. These hybrid molecules can be used deliver chemical functionality to specific sequences within deoxyribonucleic acid and ribonucleic acid. The peptides and proteins can also be used to enhance the hybridization properties of the attached oligonucleotides.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cross-Linking Reagents*
  • Micrococcal Nuclease / chemistry
  • Oligonucleotides / chemical synthesis
  • Oligonucleotides / chemistry*
  • Peptides / chemistry*
  • Proteins / chemistry*
  • Sulfhydryl Compounds / chemical synthesis

Substances

  • Cross-Linking Reagents
  • Oligonucleotides
  • Peptides
  • Proteins
  • Sulfhydryl Compounds
  • Micrococcal Nuclease